The hippocampus is crucial in episodic memory, including events associated with different spatial values. Reactivating place cells during sharp-wave ripples (SWRs) contributes to memory consolidation by transferring information from the hippocampus to neocortical networks. Previous studies primarily focused on the dorsal hippocampus (dHP), where place cells replay recent navigational traces or upcoming paths leading to goal locations during SWRs. However, whether different place-associated values influence these reactivations remains largely unknown.

In our study, we simultaneously recorded neural activities from single units in both the dHP and intermediate hippocampus (iHP) while rats performed a place-preference task on a T-maze. Notably, place cells in the iHP reactivated more for high-value locations, whereas the dHP replayed precise spatial paths leading to high-value rewards. The iHP exhibited minimal sequential reactivation of spatial representations related to high- or low-value locations. Despite this, its larger place fields may make it better suited for representing spatial values. Meanwhile, in the dHP, approximately 85% of replay events represented paths to higher-value locations, consistent with prior research favoring high-value rewards. Our findings suggest that functionally diverse information reactivation occurs in different hippocampal regions, the iHP and dHP, when goal-directed navigation requires distinguishing places based on their values.